Fig. three demonstrates an case in point wherein the yellow element has a powerful affinity Along with the cell section and moves quickly via the column, although the pink element has a strong affinity With all the stationary stage and moves as a result of slowly. The elution speed in the column depends on the affinity in between the compound as
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Interference may be overcome by appropriate procedure, like filtration, neutralization, dialysis, or heating. To ascertain that the chosen treatment method effectively eradicates interference without having loss of endotoxins, complete the assay explained below utilizing the preparing for being examined to which USP Endotoxin RS is additional and t
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